ISO. First edition. Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation. ISO () Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of. Buy ISO , Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices, Part 1, at CLSI.
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Breakpoints for short incubation Expert rules and intrinsic resistance Resistance mechanisms Guidance documents Consultations – New! In this multicenter study we address this issue by comparing the concentrations of hydrophilic and oso antifungal agents, represented by fluconazole FLC and itraconazole ITCrespectively, in broth microdilution plates prepared according to the ISO standard as well as by serial dilution procedures.
ISO Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices — Part 2: Regarding serial 2-fold dilution procedures, better results were detected when FLC plates were prepared according to the hydrophilic rather than the hydrophobic way.
Where clinical data is lacking! FLC eluted at 4. In order to determine and compare the accuracy of each method described for microdilution, two consecutive Ido determinations of the azole concentration were calculated from different wells of trays prepared according ISO recommendations, serial dilutions for hydrophobic drugs, and serial dilutions for hydrophilic compounds.
ITC is a weakly basic drug pKa ca. While this is the gold standard for 02776 of dilution series, the question has risen if modern pipettes are sufficiently 2077 to allow dilution series to be prepared as serial dilutions without a loss of accuracy.
Example of a quick liquid chromatographic assay of itraconazole in serum. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi, 2nd ed. ISO Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices – Part 1: Notably, although relative errors were greater for the lowest concentrations, we did not observe an increasing inaccuracy when performing serial dilutions compared to dilutions performed following the ISO procedure as would have been expected if lso precision of modern pipettes had been insufficient.
Considering only data for dilution series prepared in Spain, no systematic difference was detected between ISO dilution and serial dilution. Comparisons between results obtained by the tray preparation method were done by using analysis of variance ANOVA. Previous versions of documents Veterinary Susceptibility Testing Our data showed similar precision 207776 accuracy within each laboratory of the three procedures used for FLC microplate preparation.
Furthermore, previous studies have 27076 a much higher interlaboratory variation in MIC results for quality control strains for itraconazole than for flucytosine and fluconazole 6 – 8. Abstract A multicenter study was conducted to assess the accuracy of the ISO standard and the serial 2-fold dilution procedures for antifungal susceptibility testing.
Contact Sitemap Privacy Statement Disclaimer. Open in a separate window. Reference method ieo testing the in vitro activity of izo agents against rapidly growing anaerobic bacteria involved in infectious diseases. Author information Article notes Copyright and License information Disclaimer. General Committee to Clinical and Laboratory Standards Institute.
Precision of itraconazole dilution series by procedure and laboratory a. Sensitive determination of itraconazole and its active metabolite in human plasma by column-switching high-performance liquid chromatography with ultraviolet detection. Sitemap Search close sitemap. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility testing devices.
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Itraconazole formulation studies of the melt-extrusion process with mixture design. Quality control procedures of AST should be implemented in order to minimize the inaccuracy of dilution methods and lack of reproducibility when hydrophobic compounds such as ITC are tested.
Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. Precision of fluconazole dilution series by dilution procedure and laboratory a. Selective high-performance liquid chromatographic assay for itraconazole and hydroxyitraconazole in plasma 20767 human immunodeficiency virus-infected patients.
First indications of improved capability and capacity of public health microbiology laboratories in the European Union http: Meetings, Minutes and Reports According to those standards and the ISO recommendations, the 2-fold dilutions of compounds should 207766 performed in a multistep, rather complex, and time-consuming fashion, avoiding serial dilutions in order to prevent the risk of izo systematic error being induced, for instance, by an imprecise pipette 9.
In ido, our data suggest that microdilution trays for hydrophilic drugs such as FLC can be accurately prepared following the ISO recommendations or the serial 2-fold dilution schemes. When there are no breakpoints?
ECDC roadmap for integration of molecular typing and genomic typing into European-level surveillance and epidemic preparedness — Version 2. International Organization for Standards.